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KMID : 0382619850050010297
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Hanyang Journal of Medicine
1985 Volume.5 No. 1 p.297 ~ p.308
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Pyroninophilic Granules in the Hepatic Parenchymal Cells of Mice Treated with Mitomycin
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Abstract
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Mitomycin, one of the mitosane antibiotics, is isolated from Streptomyces caespitosus which has anticancer effect. It is well known that mitomycin inhibites DNA synthesis in mammalian cells. The major machanism accounting for the inhibitory effect of mitomycin is interference of the template activities of the DNA by cross link-ing to DNA double helix.
The auther has investigated the effect of mitomycin on the liver of mice, observ-ing the distribution and disaggregation of the pyroninophilic granules in the hepatic parenchymal cells.
A total of 52 healthy mice of ICR strain (the weight 20g) were divided into two groups; The control and the mitomycin treated groups. All experimental animals were given food and water ad libitum. The animals of the mitomycin treated groups were administered 4mg per kg of body weight of mitomycin diluted in the water for injec-tion by intraperitoneal injection. The animals of the MC treated groups were sacrified at intervals of 6, 12, 24 and 36 hours after administration of mitomycin Histochemical preparations were stained with the methyl green-pyronin method for the pyroninophilic granules in the hepatic parenchymal cells.
A marked reduction or disappearance of the pyroninophilic granules were observed in the entire hepatic lobule at 24 hours after administration of mitomycin.
Consequently, it is suggested that mitomycin causes the loss of pyroninophilic granules in the hepatic parenchymal cells of the liver in the mitomycin treated mice.
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